Thaw the ladder either at room temperature or at 37-40☌ for a few minutes to dissolve precipitated solids.
#Bio rad marker protein verification#
The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).ĥ00 µL Protein Marker sufficient for 100 mini gels or 50 large gels. Precision Plus Protein standards for protein electrophoresis contain ten prestained, highly purified recombinant proteins with molecular masses from 10 to 250. polymerase with a T100 thermal cycler (Bio-Rad, Hercules, CA, USA). Protein Markers Protein molecular weight markers, sometimes referred to as protein standards or protein ladders, are used to estimate the molecular weight of proteins of interest and to monitor the progress of electrophoretic separation or transfer in Western blotting. before being passed through a hydrophobic chromatography column (Bio-Rad Beads SM-2). The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240 kDa. To fine map the Rsps gene, InDel markers were designed within the Rsps target region. Denatured protein was obtained in a single step using a Ni-column. Details BlueStar PLUS Prestained Protein Marker Molecular mass marker proteins: sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) standard (Bio-Rad).
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